Isolation and Characterization of Edestin from Cheungsam Hempseed

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Isolation and Characterization of Edestin from Cheungsam Hempseed

Edestin, a major hempseed storage protein from Cheungsam, was isolated to apparent homogeneity by acid precipitation and gel filtration chromatography. The native molecular weight of purified edestin was approximately 300 kDa by Sephacryl S-300 gel filtration. Upon adding 2mercaptoethanol, the isolated edestin of 56.7 kDa on the non-reduced sodium dodecyl sulfate polyacrylamide gel was converte...

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ژورنال

عنوان ژورنال: Journal of Applied Biological Chemistry

سال: 2011

ISSN: 1976-0442

DOI: 10.3839/jabc.2011.015